Native Proteins

Topoisomerase II alpha | Topoisomerase II alpha Assay Kit | FACT | SAE1/SAE2 | In Vitro Sumoylation Kit | HeLa Core Histones | In Vitro Chromatin Assembly Kit | HnRNP U | Nucleophosmin/B23 | Nucleolin | Ku70/86  | HSP90

larger image		 Human topoisomerase II alpha
  • Cat No: T-001
  • Price: $ 249
  • Size: 200 units*
  • Purity: >95%
  • Intactness: >95%
  • Stability: No detectable change in activity up to 12 months of storage at -70oC and after 20 cycles of freeze-thawing.
Key features: Highly active protein purified from HeLa cells. Specific activity is approximately 500K units per mg protein. *1 unit is defined as the activity to catalyze decatenation of 250 ng of subtrate DNA in 15 min at 37oC. In addition to highly active human topoisomerase II alpha, the following reagents will be provided.
  • 10x Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (2 mg/ml, 250 ul)

larger image		 Human topoisomerase II alpha
  • Cat No: T-002
  • Price: $ 999
  • Size: 1000 units*
  • Purity: >95%
  • Intactness: >95%
  • Stability: No detectable change in activity up to 12 months of storage at -70oC and after 20 cycles of freeze-thawing.
Key features: Highly active protein purified from HeLa cells. Specific activity is approximately 500K units per mg protein. *1 unit is defined as the activity to catalyze decatenation of 250 ng of catenated DNA in 15 min at 37oC. In addition to highly active human topoisomerase II alpha, the following reagents will be provided.
  • 10x Reaction Buffer (1250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M  MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (1250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (2 mg/ml, 1250 ul)

larger image		 Topoisomerase II alpha assay Kit
  • Cat No: TA-001
  • Price: $ 349
  • Size: 100 reactions
Key features: In addition to highly active human topoisomerase II alpha, this assay kit contains the following reagents.
  • 10x Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (250 ul, 2 mg/ml)
  • Topoisomerase II alpha (100 units)
  • Catenated kinetoplast DNA (25 ug)
  • 6% SDS (250 ul)
  • 3 mg/ml Proteinase-K (250 ul)
  • 5x Loading buffer (500 ul)
For detailed info, download topo-manual.

larger image		 Human FACT
  • Cat No: F-001
  • Price: $ 249
  • Size: 2 ug
  • Purity: >95%
  • Intactness: >95%
Key features: Human native heterodimeric complex purified from HeLa cells.

larger image		 Human FACT
  • Cat No: F-002
  • Price: $ 999
  • Size: 10 ug
  • Purity: >95%
  • Intactness: >95%
Key features: Human native heterodimeric complex purified from HeLa cells.

larger image		 Human SAE1/SAE2
  • Cat No: S-001
  • Price: $ 349
  • Size: 100 units*
  • Purity: >95%
  • Intactness: >95%
Key features: Highly active heterodimeric protein purified from HeLa cells. Specific activity is approximately 67K units per mg protein. *1 unit is defined as the activity to catalyze sumoylation of 400 pmol of substrate peptide in 120 min at 37oC.

larger image		 In vitro Sumoylation Kit
  • Cat No: SA-001
  • Price: $ 499
  • Size: 50 reactions
Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, and 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Gst-IkBa, 50 ul, 0.5 mg/ml)
  • 5x SDS sample buffer
For detailed info, download sumo-manual.

larger image		 In vitro Sumoylation Kit
  • Cat No: SB-001
  • Price: $ 499
  • Size: 50 reactions
Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.  
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Flag/Gst-RHA137, 50 ul, 0.5 mg/ml)
  • 5x SDS sample buffer (250 ul)
For detailed info, download sumo-manual.

larger image		 HeLa core histones
  • Cat No: H-001
  • Price: $ 249
  • Size: 100 ug
  • Purity: >95%
  • Intactness: >95%
Key features: Isolated from HeLa nuclei. Highly active in assays such as histone acetylation and chromatin assembly. In addition to core histones, BSA (100 ug, 2 mg/ml) will be provided as a control protein.

larger image		 In vitro Chromatin Assembly Kit
  • Cat No: HA-001
  • Price: $ 499
  • Size: In vitro chromatinization of up to 100 ug of DNA.
  • Efficiency: >95%
Key features: The use of an internal control DNA makes it possible to monitor the efficiency of in vitro chromatin assembly with target DNA of either linear or circular conformation. In vitro chromatin assembly kit contains the following reagents:
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 0.1 mM EDTA, 0.5 mM PMSF, and 10 mM DTT.
  • Control DNA template (pNAS, 2 ug, 0.1 mg/ml)
  • PCR primer mix (40 ul, 100 pmol/ul)
  • 5 M NaCl (200 ul)
  • HeLa core histones (50 ul, 2 mg/ml)
  • Dilution Buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 0.1 mM EDTA, 0.5 mM PMSF, and 1 mM DTT
  • Storage Buffer (1 ml): 40 mM Hepes-NaOH, pH 7.4, 0.2 mM EDTA, 0.5 mM PMSF, 2 mM DTT, 0.1% NP-40, 0.6 mg/ml BSA, and 30% glycerol.
For detailed info, download cak-manual.



larger image		 HnRNP U
(Heterogeneous ribonucleoprotein U)
  • Cat No: U-001
  • Price: $ 249
  • Size: 5 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).


larger image		 HnRNP U
(Heterogeneous ribonucleoprotein U)
  • Cat No: U-002
  • Price: $ 999
  • Size: 25 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).


larger image
NPM (Nucleophosmin/B23)
  • Cat No: B-001
  • Price: $ 249
  • Size: 2 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



larger image
NPM (Nucleophosmin/B23)
  • Cat No: B-002
  • Price: $ 999
  • Size: 10 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).


larger image		 Nucleolin
  • Cat No: NN-001
  • Price: $ 299
  • Size: 2 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



larger image
Nucleolin
  • Cat No: NN-002
  • Price: $ 1199
  • Size: 10 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



larger image
 Ku70/86
  • Cat No: KU-001
  • Price: $ 349
  • Size: 2 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).



larger image
Ku70/86
  • Cat No: KU-002
  • Price: $ 1299
  • Size: 10 ug
  • Purity: >95%
Key features: Native protein isolated from nuclear extracts of HeLa suspension culture, employing ammmonium sulfate fractionation, column chromatographic steps, and ultracentrifugal separation through glycerol gradient (20-40%).


larger image

HSP90
  • Cat No: HP-001
  • Price: $ 249
  • Size: 10 ug
  • Purity: >95%
Key features: Hsp90b is a molecular chaperone regulating the function of client proteins, including signalling proteins. Hsp90b is isolated from nuclear extracts of HeLa cells through ammonium sulfate fractionation, ion-exchage column chromatographic steps utilizing Q-Sepharose, S-Sepharose, hydroxyapatite, and phosphocellulose, followed by ultracentrifugal separation on a glycerol gradient (20 to 40%). Hsp90b is highly pure and is in a native homodimeric conformation.


larger image

HSP90
  • Cat No: HP-002
  • Price: $ 999
  • Size: 50 ug
  • Purity: >95%
Key features: Hsp90b is a molecular chaperone regulating the function of client proteins, including signalling proteins. Hsp90b is isolated from nuclear extracts of HeLa cells through ammonium sulfate fractionation, ion-exchage column chromatographic steps utilizing Q-Sepharose, S-Sepharose, hydroxyapatite, and phosphocellulose, followed by ultracentrifugal separation on a glycerol gradient (20 to 40%). Hsp90b is highly pure and is in a native homodimeric conformation.

Copyright @ 2008 Vaxron Corp. All Rights Reserved.