Assay Kits

Topoisomerase II alpha Assay Kit | In Vitro Chromatin Assembly Kit | In Vitro Sumoylation Kit | Nucleolin EMSA Kit | Nucleophosmin EMSA Kit

larger image		 Topoisomerase II alpha assay Kit
  • Cat No: TA-001
  • Price: $ 349
  • Size: 100 reactions
Key features: In addition to highly active human topoisomerase II alpha, this assay kit contains the following reagents.
  • 10x Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, 0.1 M MgCl2, and 20 mM ATP.
  • 10x Control Reaction Buffer (250 ul): 0.5 M Tris-HCl, pH 8.0, 1.0 M NaCl, 10 mM DTT, and 0.1 M  MgCl2.
  • 10x BSA (250 ul, 2 mg/ml)
  • Topoisomerase II alpha (100 units)
  • Catenated kinetoplast DNA (25 ug)
  • 6% SDS (250 ul)
  • 3 mg/ml Proteinase-K (250 ul)
  • 5x Loading buffer (500 ul)
For detailed info, download topo-manual.

larger image		 In vitro Chromatin Assembly Kit
  • Cat No: HA-001
  • Price: $ 499
  • Size: In vitro chromatinization of up to 100 ug of DNA.
  • Efficiency: >95%
Key features: The use of an internal control DNA makes it possible to monitor the efficiency of in vitro chromatin assembly with target DNA of either linear or circular conformation. In vitro chromatin assembly kit contains the following reagents:
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 0.1 mM EDTA, 0.5 mM PMSF, and 10 mM DTT.
  • Control DNA template (pNAS, 2 ug, 0.1 mg/ml)
  • PCR primer mix (40 ul, 100 pmol/ul)
  • 5 M NaCl (200 ul)
  • HeLa core histones (50 ul, 2 mg/ml)
  • Dilution Buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 0.1 mM EDTA, 0.5 mM PMSF, and 1 mM DTT
  • Storage Buffer (1 ml): 40 mM Hepes-NaOH, pH 7.4, 0.2 mM EDTA, 0.5 mM PMSF, 2 mM DTT, 0.1% NP-40, 0.6 mg/ml BSA, and 30% glycerol.
For detailed info, download cak-manual.

larger image		 In vitro Sumoylation Kit
  • Cat No: SA-001
  • Price: $ 499
  • Size: 50 reactions
Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, and 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Gst-IkBa, 50 ul, 0.5 mg/ml)
  • 5x SDS sample buffer
For detailed info, download sumo-manual.

larger image		 In vitro Sumoylation Kit
  • Cat No: SB-001
  • Price: $ 499
  • Size: 50 reactions
Key features: In addition to highly active human SAE1/SAE2 (50 units), this assay kit contains the following reagents.
  • 10x Reaction Buffer (200 ul): 0.2 M Hepes-NaOH, pH 7.4, 50 mM MgCl2, 20 mM ATP, 0.5 mM PMSF, and 1 mg/ml BSA.  
  • SUMO-1 (50 ul, 1 mg/ml)
  • GST-UBC9 (50 ul, 0.4 mg/ml)
  • Control peptide (Flag/Gst-RHA137, 50 ul, 0.5 mg/ml)
  • 5x SDS sample buffer (250 ul)
For detailed info, download sumo-manual.

larger image		 Nucleolin EMSA Kit
  • Cat No: NS-001
  • Price: $ 649
  • Size: 100 reactions
Key features: Nucleolin, isolated from HeLa cells, harbors an activity to efficiently interact with RNA in vitro, without changing the overall integrity of substrate RNA. In a standard reaction (10 ul), nucleolin (50 ng) forms complexes with virtually all RNA substrates (1 nM) provided. However, the optimal concentration of nucleolin should be emperically determined for each RNA substrate of researcher's interest. The following reagents will be provided:
  • Nucleolin: 5 ug (0.1 mg/ml).
  • 10x Reaction buffer (0.25 ml): 0.2 M Hepes-NaOH, pH 7.4, 1 M NaCl, 10 mM DTT, and 2 mg/ml BSA.
  • 5x Loading buffer (0.5 ml); 0.1 M Tris-HCl, pH 7.4, 5 mM EDTA, 0.05% NP-40, 0.01% bromophenol blue, 0.01% xylene cyanol, and 50% glycerol
  • Protein dilution buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 50 mM NaCl, 0.1 mM EDTA, 0.5 mM PMSF, 0.2 mg/ml bovine serum albumin, 0.05% Noniodit P-40, a cocktail of protease inhibitors, and 20% glycerol.
  • Instruction manual.
   

larger image		 Nucleophosmin EMSA Kit
  • Cat No: BS-001
  • Price: $ 549
  • Size: 100 reactions
  • Storage: -70oC
Key features: Purified NPM is in a multimer conformation and highly active in interacting with RNA. In a standard reaction (10 ul), NPM (50 ng) forms stable complexes with virtually all RNA substrates (1 nM) provided. However, the optimal concentration of NPM1 should be emperically determined for each RNA substrate of researcher's interest. The following reagents will be provided:
  • NPM: 5 ug (0.1 mg/ml).
  • 10x Reaction buffer (0.25 ml): 0.2 M Hepes-NaOH, pH 7.4, 1 M NaCl, 10 mM DTT, and 2 mg/ml BSA.
  • 5x Loading buffer (0.5 ml): 0.1 M Tris-HCl, pH 7.4, 5 mM EDTA, 0.05% NP-40, 0.01% bromophenol blue, 0.01% xylene cyanol, and 50% glycerol
  • Protein dilution buffer (0.5 ml): 20 mM Hepes-NaOH, pH 7.4, 50 mM NaCl, 0.1 mM EDTA, 0.5 mM PMSF, 0.2 mg/ml bovine serum albumin, 0.05% Noniodit P-40, a cocktail of protease inhibitors, and 20% glycerol.
  • Insturction manual.
   

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